Proteome of monoclonal antibody-purified haustoria from Puccinia triticina Race-1.
Identifieur interne : 000091 ( Main/Exploration ); précédent : 000090; suivant : 000092Proteome of monoclonal antibody-purified haustoria from Puccinia triticina Race-1.
Auteurs : Christof Rampitsch [Canada] ; Asl Han Günel ; Eva Beimcik ; Wayne MautheSource :
- Proteomics [ 1615-9861 ] ; 2015.
Descripteurs français
- KwdFr :
- Anticorps monoclonaux (composition chimique), Basidiomycota (composition chimique), Données de séquences moléculaires (MeSH), Maladies des plantes (microbiologie), Protéines fongiques (composition chimique), Protéines fongiques (isolement et purification), Protéome (composition chimique), Protéome (isolement et purification), Protéomique (MeSH), Spectrométrie de masse en tandem (MeSH), Séquence d'acides aminés (MeSH), Triticum (microbiologie).
- MESH :
- composition chimique : Anticorps monoclonaux, Basidiomycota, Protéines fongiques, Protéome.
- isolement et purification : Protéines fongiques, Protéome.
- microbiologie : Maladies des plantes, Triticum.
- Données de séquences moléculaires, Protéomique, Spectrométrie de masse en tandem, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Antibodies, Monoclonal (chemistry), Basidiomycota (chemistry), Fungal Proteins (chemistry), Fungal Proteins (isolation & purification), Molecular Sequence Data (MeSH), Plant Diseases (microbiology), Proteome (chemistry), Proteome (isolation & purification), Proteomics (MeSH), Tandem Mass Spectrometry (MeSH), Triticum (microbiology).
- MESH :
- chemical , chemistry : Antibodies, Monoclonal, Fungal Proteins, Proteome.
- chemistry : Basidiomycota.
- chemical , isolation & purification : Fungal Proteins, Proteome.
- microbiology : Plant Diseases, Triticum.
- Amino Acid Sequence, Molecular Sequence Data, Proteomics, Tandem Mass Spectrometry.
Abstract
Puccinia triticina causes leaf rust, a disease that causes annual yield losses in wheat. It is an obligate parasite that invades the host leaf and forms intracellular structures called haustoria, which obtain nutrients and suppress host immunity using secreted proteins called effectors. Since effector proteins act at the frontier between plant and pathogen and help determine the outcome of the interaction, it is critical to understand their functions. Here, we used a direct proteomics approach to identify effector candidates from P. triticina Race 1 haustoria isolated with a specific monoclonal antibody. Haustoria were >95% pure and free of host contaminants. Using high resolution MS we have identified 1192 haustoria proteins. These were quantified using normalized spectral counts and spanned a dynamic range of three orders of magnitude, with unknown proteins and metabolic enzymes as the most highly represented. The dataset contained 140 candidate effector proteins, based on the presence of a signal peptide and the absence of a known function for the protein. Some of these candidates were significantly enriched with cysteine, with up to 13 residues per protein and up to 6.8% cysteine in composition.
DOI: 10.1002/pmic.201400241
PubMed: 25546510
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Triticum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Molecular Sequence Data</term><term>Proteomics</term><term>Tandem Mass Spectrometry</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Données de séquences moléculaires</term><term>Protéomique</term><term>Spectrométrie de masse en tandem</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Puccinia triticina causes leaf rust, a disease that causes annual yield losses in wheat. It is an obligate parasite that invades the host leaf and forms intracellular structures called haustoria, which obtain nutrients and suppress host immunity using secreted proteins called effectors. Since effector proteins act at the frontier between plant and pathogen and help determine the outcome of the interaction, it is critical to understand their functions. Here, we used a direct proteomics approach to identify effector candidates from P. triticina Race 1 haustoria isolated with a specific monoclonal antibody. Haustoria were >95% pure and free of host contaminants. Using high resolution MS we have identified 1192 haustoria proteins. These were quantified using normalized spectral counts and spanned a dynamic range of three orders of magnitude, with unknown proteins and metabolic enzymes as the most highly represented. The dataset contained 140 candidate effector proteins, based on the presence of a signal peptide and the absence of a known function for the protein. Some of these candidates were significantly enriched with cysteine, with up to 13 residues per protein and up to 6.8% cysteine in composition. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25546510</PMID><DateCompleted><Year>2015</Year><Month>12</Month><Day>28</Day></DateCompleted><DateRevised><Year>2015</Year><Month>04</Month><Day>01</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1615-9861</ISSN><JournalIssue CitedMedium="Internet"><Volume>15</Volume><Issue>7</Issue><PubDate><Year>2015</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Proteomics</Title><ISOAbbreviation>Proteomics</ISOAbbreviation></Journal><ArticleTitle>Proteome of monoclonal antibody-purified haustoria from Puccinia triticina Race-1.</ArticleTitle><Pagination><MedlinePgn>1307-15</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/pmic.201400241</ELocationID><Abstract><AbstractText>Puccinia triticina causes leaf rust, a disease that causes annual yield losses in wheat. It is an obligate parasite that invades the host leaf and forms intracellular structures called haustoria, which obtain nutrients and suppress host immunity using secreted proteins called effectors. Since effector proteins act at the frontier between plant and pathogen and help determine the outcome of the interaction, it is critical to understand their functions. Here, we used a direct proteomics approach to identify effector candidates from P. triticina Race 1 haustoria isolated with a specific monoclonal antibody. Haustoria were >95% pure and free of host contaminants. Using high resolution MS we have identified 1192 haustoria proteins. These were quantified using normalized spectral counts and spanned a dynamic range of three orders of magnitude, with unknown proteins and metabolic enzymes as the most highly represented. The dataset contained 140 candidate effector proteins, based on the presence of a signal peptide and the absence of a known function for the protein. 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Günel</name><name sortKey="Mauthe, Wayne" sort="Mauthe, Wayne" uniqKey="Mauthe W" first="Wayne" last="Mauthe">Wayne Mauthe</name></noCountry><country name="Canada"><noRegion><name sortKey="Rampitsch, Christof" sort="Rampitsch, Christof" uniqKey="Rampitsch C" first="Christof" last="Rampitsch">Christof Rampitsch</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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